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puc118 bam hi bap vector  (TaKaRa)


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    Structured Review

    TaKaRa puc118 bam hi bap vector
    Puc118 Bam Hi Bap Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc+118+vector/pUC+118+BamH+I%2FBAP/pmc10999558-81-8-12
    Average 94 stars, based on 27 article reviews
    puc118 bam hi bap vector - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Isolation of a germin-like protein with manganese superoxide dismutase activity from cells of a moss, Barbula unguiculata.
    Article Snippet: The cDNA library was screened with the PCR product, which was labeled with digoxigenin using a digoxigenin DNA labeling and detection kit (Roche Molecular Biochemicals). .. Inserts from the moss cDNA clones that hybridized to digoxigenin-labeled DNA probes were extracted from recombinant phage, subcloned into the pUC 118 vector (TaKaRa Shuzo, Kyoto, Japan), and sequenced on both strands. .. Nucleotide Sequence Analysis—Sequencing was carried out by the dideoxy chain termination method using Thermo Sequenase dye terminator sequencing premix kit, version 2.0 (Amersham Pharmacia Biotech) and with a DNA sequencer (model 373A, Applied Biosystems, CA).

    Article Title: Emergence of an erythroid cell-specific regulatory region in ABO intron 1 attributable to A- or B-antigen expression on erythrocytes in Hominoidea
    Article Snippet: The sequencing run was performed on a genetic analyzer (Thermo Fisher Scientific, Seqstudio). .. Also, the PCR2 products were ligated into the pUC 118 vector using a Mighty Cloning Reagent set (Blunt End) (TaKaRa), followed by determination of the nucleotide sequences of the clones obtained using the primers M13M4 and M13RV. ..

    Article Title: Sequence analysis of ABO and its homologues is valid for species identification.
    Article Snippet: Background: ABO and its paralogues, such as A3GALT2 and GGTA1, encoding α1,3-Gal(NAc) transferases, belong to the glycosyltransferase 6 (GT6) gene family.. We have developed an alternative method for the identification of species based on sequence variations within the GT6 gene family, which is applicable to degraded DNA.. Methods/Materials: DNA samples prepared from control mammalian species, together with an unknown sample, were polymerase chain reaction (PCR)-amplified using one universal primer pair targeting the sequences in the last coding exons of the GT6 gene family, yielding 141-bp products derived from those multiple loci.

    Article Title: Molecular characterization and phylogenetic analysis of Fasciola gigantica from Nigeria.
    Article Snippet: Article history: Received 11 June 2016 Received in revised form 30 August 2016 Accepted 18 October 2016 Available online 19 October 2016 Fasciola gigantica is considered the major pathogen causing fasciolosis in Africa; however, molecular characterization of this fluke has not been adequately elucidated.. It is important to scientifically elucidate the dispersal history of F. gigantica by analyzing its genetic diversity.. Fasciola flukes fromNigeriawere analyzed using nuclear and mitochondrial DNAmarkers.

    Recombinant:

    Article Title: Isolation of a germin-like protein with manganese superoxide dismutase activity from cells of a moss, Barbula unguiculata.
    Article Snippet: The cDNA library was screened with the PCR product, which was labeled with digoxigenin using a digoxigenin DNA labeling and detection kit (Roche Molecular Biochemicals). .. Inserts from the moss cDNA clones that hybridized to digoxigenin-labeled DNA probes were extracted from recombinant phage, subcloned into the pUC 118 vector (TaKaRa Shuzo, Kyoto, Japan), and sequenced on both strands. .. Nucleotide Sequence Analysis—Sequencing was carried out by the dideoxy chain termination method using Thermo Sequenase dye terminator sequencing premix kit, version 2.0 (Amersham Pharmacia Biotech) and with a DNA sequencer (model 373A, Applied Biosystems, CA).

    Plasmid Preparation:

    Article Title: Isolation of a germin-like protein with manganese superoxide dismutase activity from cells of a moss, Barbula unguiculata.
    Article Snippet: The cDNA library was screened with the PCR product, which was labeled with digoxigenin using a digoxigenin DNA labeling and detection kit (Roche Molecular Biochemicals). .. Inserts from the moss cDNA clones that hybridized to digoxigenin-labeled DNA probes were extracted from recombinant phage, subcloned into the pUC 118 vector (TaKaRa Shuzo, Kyoto, Japan), and sequenced on both strands. .. Nucleotide Sequence Analysis—Sequencing was carried out by the dideoxy chain termination method using Thermo Sequenase dye terminator sequencing premix kit, version 2.0 (Amersham Pharmacia Biotech) and with a DNA sequencer (model 373A, Applied Biosystems, CA).

    Article Title: Emergence of an erythroid cell-specific regulatory region in ABO intron 1 attributable to A- or B-antigen expression on erythrocytes in Hominoidea
    Article Snippet: The sequencing run was performed on a genetic analyzer (Thermo Fisher Scientific, Seqstudio). .. Also, the PCR2 products were ligated into the pUC 118 vector using a Mighty Cloning Reagent set (Blunt End) (TaKaRa), followed by determination of the nucleotide sequences of the clones obtained using the primers M13M4 and M13RV. ..

    Article Title: Sequence analysis of ABO and its homologues is valid for species identification.
    Article Snippet: Background: ABO and its paralogues, such as A3GALT2 and GGTA1, encoding α1,3-Gal(NAc) transferases, belong to the glycosyltransferase 6 (GT6) gene family.. We have developed an alternative method for the identification of species based on sequence variations within the GT6 gene family, which is applicable to degraded DNA.. Methods/Materials: DNA samples prepared from control mammalian species, together with an unknown sample, were polymerase chain reaction (PCR)-amplified using one universal primer pair targeting the sequences in the last coding exons of the GT6 gene family, yielding 141-bp products derived from those multiple loci.

    Cloning:

    Article Title: Emergence of an erythroid cell-specific regulatory region in ABO intron 1 attributable to A- or B-antigen expression on erythrocytes in Hominoidea
    Article Snippet: The sequencing run was performed on a genetic analyzer (Thermo Fisher Scientific, Seqstudio). .. Also, the PCR2 products were ligated into the pUC 118 vector using a Mighty Cloning Reagent set (Blunt End) (TaKaRa), followed by determination of the nucleotide sequences of the clones obtained using the primers M13M4 and M13RV. ..

    Article Title: Sequence analysis of ABO and its homologues is valid for species identification.
    Article Snippet: Background: ABO and its paralogues, such as A3GALT2 and GGTA1, encoding α1,3-Gal(NAc) transferases, belong to the glycosyltransferase 6 (GT6) gene family.. We have developed an alternative method for the identification of species based on sequence variations within the GT6 gene family, which is applicable to degraded DNA.. Methods/Materials: DNA samples prepared from control mammalian species, together with an unknown sample, were polymerase chain reaction (PCR)-amplified using one universal primer pair targeting the sequences in the last coding exons of the GT6 gene family, yielding 141-bp products derived from those multiple loci.

    Article Title: Molecular characterization and phylogenetic analysis of Fasciola gigantica from Nigeria.
    Article Snippet: Article history: Received 11 June 2016 Received in revised form 30 August 2016 Accepted 18 October 2016 Available online 19 October 2016 Fasciola gigantica is considered the major pathogen causing fasciolosis in Africa; however, molecular characterization of this fluke has not been adequately elucidated.. It is important to scientifically elucidate the dispersal history of F. gigantica by analyzing its genetic diversity.. Fasciola flukes fromNigeriawere analyzed using nuclear and mitochondrial DNAmarkers.

    Amplification:

    Article Title: Sequence analysis of ABO and its homologues is valid for species identification.
    Article Snippet: Background: ABO and its paralogues, such as A3GALT2 and GGTA1, encoding α1,3-Gal(NAc) transferases, belong to the glycosyltransferase 6 (GT6) gene family.. We have developed an alternative method for the identification of species based on sequence variations within the GT6 gene family, which is applicable to degraded DNA.. Methods/Materials: DNA samples prepared from control mammalian species, together with an unknown sample, were polymerase chain reaction (PCR)-amplified using one universal primer pair targeting the sequences in the last coding exons of the GT6 gene family, yielding 141-bp products derived from those multiple loci.

    Incubation:

    Article Title: Sequence analysis of ABO and its homologues is valid for species identification.
    Article Snippet: Background: ABO and its paralogues, such as A3GALT2 and GGTA1, encoding α1,3-Gal(NAc) transferases, belong to the glycosyltransferase 6 (GT6) gene family.. We have developed an alternative method for the identification of species based on sequence variations within the GT6 gene family, which is applicable to degraded DNA.. Methods/Materials: DNA samples prepared from control mammalian species, together with an unknown sample, were polymerase chain reaction (PCR)-amplified using one universal primer pair targeting the sequences in the last coding exons of the GT6 gene family, yielding 141-bp products derived from those multiple loci.

    Polymerase Chain Reaction:

    Article Title: Sequence analysis of ABO and its homologues is valid for species identification.
    Article Snippet: Background: ABO and its paralogues, such as A3GALT2 and GGTA1, encoding α1,3-Gal(NAc) transferases, belong to the glycosyltransferase 6 (GT6) gene family.. We have developed an alternative method for the identification of species based on sequence variations within the GT6 gene family, which is applicable to degraded DNA.. Methods/Materials: DNA samples prepared from control mammalian species, together with an unknown sample, were polymerase chain reaction (PCR)-amplified using one universal primer pair targeting the sequences in the last coding exons of the GT6 gene family, yielding 141-bp products derived from those multiple loci.

    Article Title: Molecular characterization and phylogenetic analysis of Fasciola gigantica from Nigeria.
    Article Snippet: Article history: Received 11 June 2016 Received in revised form 30 August 2016 Accepted 18 October 2016 Available online 19 October 2016 Fasciola gigantica is considered the major pathogen causing fasciolosis in Africa; however, molecular characterization of this fluke has not been adequately elucidated.. It is important to scientifically elucidate the dispersal history of F. gigantica by analyzing its genetic diversity.. Fasciola flukes fromNigeriawere analyzed using nuclear and mitochondrial DNAmarkers.

    Purification:

    Article Title: Molecular characterization and phylogenetic analysis of Fasciola gigantica from Nigeria.
    Article Snippet: Article history: Received 11 June 2016 Received in revised form 30 August 2016 Accepted 18 October 2016 Available online 19 October 2016 Fasciola gigantica is considered the major pathogen causing fasciolosis in Africa; however, molecular characterization of this fluke has not been adequately elucidated.. It is important to scientifically elucidate the dispersal history of F. gigantica by analyzing its genetic diversity.. Fasciola flukes fromNigeriawere analyzed using nuclear and mitochondrial DNAmarkers.



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    Image Search Results


    Journal: Cell Reports Methods

    Article Title: De-erosion of X chromosome dosage compensation by the editing of XIST regulatory regions restores the differentiation potential in hPSCs

    doi: 10.1016/j.crmeth.2022.100352

    Figure Lengend Snippet:

    Article Snippet: PUC118 cloning vector , Takara Bio Inc. , Cat# 3324.

    Techniques: Recombinant, Plasmid Preparation, Lysis, Nick Translation, DNA Methylation Assay, Clone Assay, Expressing, Knock-Out, Software